Spider Silk

Nephilidae
Nephila
Important mechanical properties :
1)High tensile strength
2)High extensibility
3)High capability of water uptake
4)Biodegradability
 Bioengineering :
Method I :
1)Insert the cloned silk gene into bacteria, E. coli
2)Bacteria synthesizes the desired protein
3)Culture the bacteria for the cloned colony that would produce the synthetic polymer by way of protein synthesis
Method II :
1)Introduce silk gene in the mammary gland of goats (silk gene : a form of Recombinant DNA)
2)Clone such goats with silk gene added to their genome. These animals will form the foundation of a herd sharing identical genome. It is supposed that per litre of milk will have 2-15 gms of spider silk.
3)Silk extraction will involve conventional dairy processes, using milking machines.
4)Skim the milk for cream and whey. Once the protein fat separated by 50% filteration process, the milk will be pure enough to produce a film containing silk.
 Application of Nephila silk :
1)Used for fishing lures, traps and nets.
2)Used as crosshairs in optical targeting devices such as guns and telescopes.
3)Though successful in a small scale, spider silk synthesis if ever becomes industrially viable is thought to replace Kevlar and be used to make diverse range of items such as —
l Bulletproof clothing
lWear resistant light weight clothing
lRopes, nets, seat belts, parachutes
lRustfree panels on motor vehicles or boats
lBiodegradable bottles
lBandages, surgical thread
lArtifical tendons or ligaments, support for weak blood vessels